Journal: International Journal of Clinical and Experimental Pathology
Article Title: A possible complementary tool for diagnosing tuberculosis: a feasibility test of immunohistochemical markers
doi:
Figure Lengend Snippet: Receiver operating characteristic (ROC) curves of each of the six immunohistochemical markers, individually (A) and in combination (B): (A) ROC analysis of each of the six antibodies: The area under the curve (AUC) represents an optimal summary statistic for comparing the sensitivities and specificities of immunohistochemistry (IHC) for each of the four antibodies (see Table 4, column 3) for differentiating between tuberculous granuloma (TG) and non-tuberculous granuloma (NG). The six IHC markers are listed in descending AUC order: Lac (0.89), IDO (0.83), TNF (0.70), ESEL (0.67), FoxP3 (0.63), and TR (0.60). (B) The ROC curve of the combined predictor (designated as Combination* in Table 2), which was defined as the sum of the scores of three individual markers (LAC + IDO + negative TNF), shows increased accuracy compared to all the individual curves (AUC = 0.9, sensitivity = 66.7%, and specificity = 96.8%) and improves the test to a moderate level of accuracy. TNF = TNF-beta; ESEL = E-selectin; IDO = Indoleamine 2,3-dioxygenase; Lac = lactoferrin; TR = tartrate-resistant acid phosphatase.
Article Snippet: The antibodies used were FoxP3 (Monoclonal; 1:100; Santa Cruz Biotechnology, Dallas, TX, USA), TNF-beta (monoclonal; 1:100; Santa Cruz Biotechnology), E-selectin (ESEL) (monoclonal, 1:50; Santa Cruz Biotechnology), IDO (monoclonal, 1:50; Santa Cruz Biotechnology), lactoferrin (monoclonal, 1:50; Santa Cruz Biotechnology), and TR (monoclonal, 1:100; Abnova, Taipei, Taiwan).
Techniques: Immunohistochemical staining, Immunohistochemistry